liquid phase peptide synthesis process
liquid phase peptide synthesis process

Peptides refer to short polymers originating from interlinked amino acids. The link between different amino acid residues is generated from the reaction between a carboxyl and an amine group. This link is what you can refer to as the peptide bond.

The process of peptide formation is quite complex and consists of distinct techniques. Some of the processes involved are the solid phase synthesis and liquid phase synthesis. Before you buy peptides, delve deeper into this article to learn more about the process of peptide manufacturing.

Peptide Manufacturing Techniques

Peptide manufacturing can be done using two techniques: solid phase synthesis and liquid or solution phase synthesis. At times, a combination of both these techniques is involved in the manufacturing of peptides. Every technique will differently affect the cost and scalability of pharmaceutical products incorporating peptides.

Solid Phase Synthesis

Solid phase synthesis enables the mixing of natural peptides that are otherwise difficult to mix. It often incorporates artificially-occurring amino acids and alters peptide backbones. In this technique, amino acids help develop peptides by getting attached to polymer beads within a solution.

As long as they are not treated with a reagent, the amino acids remain attached to the polymer beads. A reagent like trifluoroacetic acid helps immobilize a peptide to make it capable of being captured through filtration.

Solid phase synthesis promotes faster production of peptides. Moreover, it enhances scalability due to its simple nature. Additionally, solid phase synthesis will help with creating longer amino acid sequences.

The solid phase synthesis method will either leverage tert-butoxycarbonyl (t-Boc) or 9H-f luoren-9-ylmethoxycarbonyl (Fmoc). T-Boc solid phase synthesis is the original method because it helps in removing Boc from a growing peptide chain under an acidic condition. This method involves using minimal quantities of hydrofluoric acid.

Using hydrofluoric acid in the process of peptide synthesis is usually considered safe for specialized equipment and regulatory requirements. The T-Boc method will support more complex peptide synthesis. In fact, it is primarily used for synthesizing unnatural peptides.

Meanwhile, the Fmoc method makes it easy to cleave peptides. The method which came into existence later also enables manufacturers to hydrolyze a peptide. By using a weaker amino acid in the Fmoc resin, the need for using special equipment gets eliminated.

Both T-Boc and FMoc methods come with special benefits and have different applications. But in recent times, the FMoc method is preferred more because it does not require the use of hydrofluoric acid.

Liquid Phase Synthesis

Liquid-based peptide synthesis is the older technique of the two. It is particularly beneficial for creating short peptide chains and helps with large-scale production. But solid phase synthesis is also becoming immensely popular for peptide manufacturing.

Ending Note

Peptides support low toxicity and high specificity and have minimal toxicology issues. Therefore, peptides are capturing the interest of pharmaceutical companies trying to produce revolutionary drugs. Lotilabs is the ultimate destination for purchasing a wide range of peptides synthesized using the best industry practices.

With high-quality and authentic peptides, they are indeed the best manufacturers and suppliers of peptides.


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